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KMID : 0900920010250040317
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Korean journal of Animal Reproduction
2001 Volume.25 No. 4 p.317 ~ p.325
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Effects of Ferrous Sulfate and Ascorbic Acid on In Vitro Fertility and Sperm Lipid Peroxidation in the Pig
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Park C.-K.
Ann J.-Y.
Kim I.-C.
Lee J.-H.
Yang B.-K.
Kim C.-I.
Cheong H.-T.
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Abstract
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This study investigated the effect of ferrous sulfate (Fe^{2+}) and/or ascorbic acid (Asc) on fertilizing ability in vitro of frozen-thawed boar spermatozoa. Using chlortetracycline (CTC) fluorescence, the spermatozoa was treated in preincubation medium with control, Fe^{2+}(1 mM), Asc (0.5 mM) and Fe^{2+}Asc to assessed for acrosome reaction, and the oocyte penetration test to determine whether the Fe^{2+} and/or Asc can promote the penetration ability in vitro. When frozen-thawed spermatozoa was washed with preincubation medium, there were significantly (P ＜ 0.05) more acrosome-reacted in medium with Fe^{2+}Asc (38%) than control (27%). The penetration rates were also significantly (P ＜ 0.05) higher in medium with Fe^{2+}Asc (76%) than control (55%). Next, the lipid peroxidation of sperm was evaluated on the basis of malondialdehyde production following same treatments. The addition of Fe^{2+}Asc to sperm suspension increases the formation of malondialdehyde. However, there were not significantly different under the all conditions. The sperm suspension were also treated with control, Fe^{2+}, Asc and Fe^{2+}/Asc and assayed for sulfhydry1(-SH) group content. In the Fe^{2+}/Asc group, sperm-SH group were higher than another groups. In spermatozoa treated with Fe^{2+} and/or Asc, however, no changes in sperm -SH-groups were detected when compared to controls. In another experiment, the activity of sperm binding to zona pellucida was evaluated through binding to salt-stored porcine oocytes. In control and Asc treatment groups, sperm binding to zona pellucida were significantly (P ＜ 0.05) higher than in medium with Fe^{2+}. On the other hand, there is not a significant increase in binding to zona pellucida with spermatozoa treated by Fe^{2+}/Asc. In summary, the present study suggests that Fe^{2+}/Asc causes an enhancement in fertilizing ability that is associated with penetration rate increased without change of spermatozoa binding capacity to homologous zona pellucida.o homologous zona pellucida.
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KEYWORD
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Ascorbic acid, Ferrous sulfate, Boar spermatozoa, in vitro fertility, Lipid peroxidation
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